Effects of Exhaustion Gases from Biodiesel Blends on the Lichen Cladonia verticillaris: A Complimentary Evaluation

The objective of this study was to evaluate the effects gases emitted by blends biodiesel with petroleum diesel at intermediate concentrations (between 10 % and 40 %) on biochemistry lichen Cladonia verticillaris (Raddi) Fr. Samples respective species were exposed during a period sixty minutes, using stationary engine, 20 (B20), 25 (B25) 30 (B30) biodiesel, from oil cotton, diesel. In 30, 60 90 days, primary metabolites (chlorophylls pheophytins) secondary (protocetraric acid fumarprotocetraric acid) determined. A control group used for comparison purposes. combustion process also evaluated three times (0 minutes minutes). results did not show significant differences chlorophylls, when all treatments materials compared, however, variations observed other metabolites. concentration protocetraric higher compared acid, indicating that there blocks in synthesis metabolic pathway. Cluster analysis showed groups B20 B25, pheophytins are related CO2, while B30 their chlorophylls NO2. demonstrate mixtures do affect integrity species, confirming recommendation use up %. Keywords: biofuels, environmental impact, living organisms, substances, biomonitoring.